Cytochrome P450 monooxygenase (CYP)

Lus piav qhia luv luv:

Hais txog CYP

ES-CYPs: Ib chav kawm ntawm redox enzymes nrog mercaptan-heme ua qhov chaw ua haujlwm rau catalyze oxygen hloov.Lawv yog cov terminal oxygenases thiab xav tau heme los qhib oxygen.

Cytochrome P450 monooxygenase catalyze oxidation, epoxidation thiab hydroxylation ntawm alkyl, hydroxylation thiab oxidation ntawm ammonia, oxidation ntawm sulfur, dealkylation (oxygen, sulfur, ammonia), oxidative dehydrogenation, deamination thiab dehalogenation.
Muaj 8 hom cytochrome P450 monooxygenase cov khoom lag luam (Ntau li ES-CYP-101～ES-CYP-108) tsim los ntawm SyncoZymes.

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Product Detail

Khoom cim npe

Hais txog CYP:

Catalytic tshuaj tiv thaiv hom:

Product Information:

Enzymes	Cov khoom siv tshuaj ntsuam xyuas (SynKit)	Specification
Enzyme hmoov	ES-CYP-101 ~ ES-CYP-108	ib txheej ntawm 8 Cytochrome P450 Monooxygenases, 50 mg txhua 8 yam khoom * 50mg / khoom, lossis lwm yam
Cov khoom siv tshuaj ntsuam xyuas (SynKit)	ES-CYP-800	ib txheej ntawm 8 Cytochrome P450 Monooxygenases, 1mg txhua 8 yam khoom * 1mg / khoom

Qhov zoo:

★ Broad substrate spectrum.
★ Kev hloov pauv siab.
★ Tsawg los ntawm cov khoom lag luam.
★ Cov tshuaj tiv thaiv me me.
★ Ib puag ncig tus phooj ywg.

Cov lus qhia rau kev siv:

➢ Nquag, cov tshuaj tiv thaiv yuav tsum muaj xws li substrate, tsis muaj tshuaj (Cov tshuaj tiv thaiv zoo pH ntawm enzyme), coenzyme (NAD(H) los yog NADP(H)), coenzyme regeneration system (xws li qabzib thiab qabzib dehydrogenase) thiab ES-CYP.coenzyme thiab coenzyme regeneration system tuaj yeem hloov tau los ntawm hydrogen peroxide hauv cov tshuaj tiv thaiv ib nrab.
➢ Txhua yam ntawm ES-CYPs cuam tshuam rau ntau yam kev xav tau zoo yuav tsum tau kawm ib tus zuj zus.
➢ High concentration Substrate los yog khoom nrog tej zaum yuav inhibit ES-CYP cov dej num.Txawm li cas los xij, qhov inhibition tuaj yeem daws tau los ntawm batch ntxiv ntawm substrate.

Piv txwv daim ntawv thov:

Piv txwv 1⁽¹⁾:

Piv txwv 2⁽²⁾:

Piv txwv 3⁽³⁾:

Piv txwv 4⁽⁴⁾:

Cia:

Khaws 2 xyoos qis dua -20 ℃.

Nco ntsoov:

Tsis txhob tiv tauj nrog huab cua xws li: kub siab, siab / qis pH thiab siab concentration organic hnyav.

Cov ntaub ntawv:

1. Zaretzki J, Matlock M, thiab Swamidass S JJ Chem.Inf.Qauv, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., et tal.J. Am.Chem.Soc., 2006, 128 (26), 8374–8375.
3. Cryle MJ., Matovic NJ., and De Voss J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., et al.Biochim.Biophys.Ib., 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK et al.Acta Crystallogr.Xyoo 2009, 65, 372.