Cytochrome P450 monooxygenase (CYP)

Kev Piav Qhia Luv:

Txog CYP

ES-CYPs: Ib pawg ntawm cov enzymes redox nrog mercaptan-heme ua lub hauv paus tseem ceeb los ua kom cov pa oxygen hloov pauv. Lawv yog cov oxygenases kawg thiab xav tau heme los ua kom cov pa oxygen ua haujlwm.

Cytochrome P450 monooxygenase catalyze qhov oxidation, epoxidation thiab hydroxylation ntawm alkyl, hydroxylation thiab oxidation ntawm ammonia, oxidation ntawm sulfur, dealkylation (oxygen, sulfur, ammonia), oxidative dehydrogenation, deamination thiab dehalogenation.
Muaj 8 hom cytochrome P450 monooxygenase khoom (Tus lej li ES-CYP-101 ～ ES-CYP-108) tsim los ntawm SyncoZymes.

Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526

Email:lchen@syncozymes.com

Xa email rau peb

Cov Khoom Qhia Txog Khoom

Cov Cim Npe Khoom

Txog CYP:

Hom tshuaj tiv thaiv catalytic:

Cov Ntaub Ntawv Khoom:

Cov Enzymes	Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit)	Cov Lus Qhia Tshwj Xeeb
Hmoov Enzyme	ES-CYP-101~ ES-CYP-108	ib pawg ntawm 8 Cytochrome P450 Monooxygenases, 50 mg txhua yam 8 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas
Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit)	ES-CYP-800	ib pawg ntawm 8 Cytochrome P450 Monooxygenases, 1mg txhua 8 yam khoom * 1mg / yam khoom

Cov txiaj ntsig:

★ Cov spectrum dav dav.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.

Cov lus qhia rau kev siv:

➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov khoom siv substrate, cov kua buffer (Qhov pH zoo tshaj plaws ntawm cov enzyme), coenzyme (NAD(H) lossis NADP(H)), coenzyme regeneration system (piv txwv li glucose thiab glucose dehydrogenase) thiab ES-CYP. coenzyme thiab coenzyme regeneration system tuaj yeem hloov los ntawm hydrogen peroxide hauv ib feem ntawm lub kaw lus tshuaj tiv thaiv.
➢ Yuav tsum kawm txog txhua yam ES-CYPs uas sib raug rau ntau yam xwm txheej zoo tshaj plaws ntawm kev ua haujlwm ib leeg.
➢ Cov khoom siv uas muaj cov concentration siab heev yuav ua rau ES-CYP ua haujlwm tsis tau. Txawm li cas los xij, qhov kev txwv no yuav raug txo los ntawm kev ntxiv cov khoom siv ntau ntau.

Piv txwv ntawm daim ntawv thov:

Piv txwv 1⁽¹⁾:

Piv txwv 2⁽²⁾:

Piv txwv 3⁽³⁾:

Piv txwv 4⁽⁴⁾:

Kev Khaws Cia:

Khaws cia rau 2 xyoos hauv qab -20 ℃.

Ceeb Toom:

Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab / qis thiab cov kuab tshuaj organic siab.

Cov ntaub ntawv siv los ua piv txwv:

1. Zaretzki J, Matlock M, thiab Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., et tal. J. Am. Chem. Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., thiab De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., et al. Biochim. Biophys. Ib., 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK et al. Acta Crystallogr. Xyoo 2009, 65, 372.