Immobilized CALB
CALB yog immobilized los ntawm lub cev adsorption ntawm lub siab hydrophobic resin uas yog macroporous, styrene / methacrylate polymer.Immobilized CALB yog qhov tsim nyog rau cov ntawv thov hauv cov kuab tshuaj organic thiab cov kuab tshuaj tsis muaj kuab lom, thiab tuaj yeem rov ua dua thiab rov siv tau ntau zaus hauv cov xwm txheej tsim nyog.
Product Code: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★ Kev ua haujlwm siab dua, kev xaiv chiral ntau dua thiab ruaj khov dua.
★ Kev ua tau zoo dua hauv cov theem tsis muaj dej.
★ Yooj yim tshem tawm los ntawm cov tshuaj tiv thaiv kab mob, tshem tawm cov tshuaj tiv thaiv sai, thiab zam cov protein seem hauv cov khoom.
★ tuaj yeem rov siv dua thiab rov siv dua los txo tus nqi.
| Kev ua si | ≥10000PLU/g |
| pH ntau rau cov tshuaj tiv thaiv | 5-9 |
| Kub ntau rau cov tshuaj tiv thaiv | 10-60 ℃ |
| Qhov tshwm sim | CALB-IMMO100-A: Lub teeb daj rau xim av CALB-IMMO100-B: Dawb rau lub teeb xim av |
| Particle loj | 300-500μm |
| Poob rau ziab ntawm 105 ℃ | 0.5% -3.0% |
| Resin rau immobilization | Macroporous, styrene / methacrylate polymer |
| Reaction hnyav | Dej, organic hnyav, thiab lwm yam, los yog tsis muaj hnyav.Rau cov tshuaj tiv thaiv hauv qee cov kuab tshuaj organic, 3% dej tuaj yeem ntxiv los txhim kho cov tshuaj tiv thaiv |
| Particle loj | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Chav tsev txhais: 1 unit sib haum mus rau synthesis ntawm 1μmol ib feeb propyl laurate los ntawm lauric acid thiab 1-propanol ntawm 60 ℃.Qhov saum toj no CALB-IMMP100-A thiab CALB-IMMO100-B sib haum mus rau immobilized nqa nrog ntau qhov sib txawv.
1. Reactor hom
Lub immobilized enzyme muaj feem xyuam rau ob qho tib si kettle batch reactor thiab tsau txaj nruam ntws reactor.Nws yuav tsum raug sau tseg kom tsis txhob crushing vim yog sab nraud quab yuam thaum pub mis los yog txhaws.
2. Cov tshuaj tiv thaiv pH, kub thiab hnyav
Lub immobilized enzyme yuav tsum tau ntxiv kawg, tom qab lwm cov ntaub ntawv ntxiv thiab yaj, thiab pH kho.
Yog hais tias kev noj cov substrate los yog tsim cov khoom yuav ua rau cov kev hloov ntawm pH thaum lub sij hawm cov tshuaj tiv thaiv, txaus buffer yuav tsum tau ntxiv rau cov tshuaj tiv thaiv system, los yog cov pH yuav tsum tau saib xyuas thiab kho thaum lub sij hawm cov tshuaj tiv thaiv.
Tsis pub dhau qhov ntsuas kub ntawm CALB (qis dua 60 ℃), qhov hloov pauv tau nce nrog qhov nce ntawm qhov kub thiab txias.Hauv kev siv tswv yim, cov tshuaj tiv thaiv kub yuav tsum raug xaiv raws li kev ruaj ntseg ntawm cov substrate lossis cov khoom.
Feem ntau, ester hydrolysis cov tshuaj tiv thaiv yog tsim nyob rau hauv aqueous theem system, thaum lub ester synthesis cov tshuaj tiv thaiv yog tsim nyob rau hauv organic theem system.Cov kuab tshuaj organic tuaj yeem yog ethanol, tetrahydrofuran, n-hexane, n-heptane thiab toluene, lossis cov kuab tshuaj sib xyaw haum.Rau cov tshuaj tiv thaiv hauv qee cov kuab tshuaj organic, 3% dej tuaj yeem ntxiv los txhim kho cov tshuaj tiv thaiv.
3. Rov siv dua thiab kev pabcuam lub neej ntawm CALB
Raws li qhov tsim nyog cov tshuaj tiv thaiv, CALB tuaj yeem rov qab tau thiab rov qab siv dua, thiab lub sijhawm thov tshwj xeeb sib txawv nrog cov haujlwm sib txawv.
Yog tias cov CALB rov qab tsis tau rov qab siv tas li thiab yuav tsum tau khaws cia tom qab rov qab los, nws yuav tsum tau ntxuav thiab qhuav thiab kaw ntawm 2-8 ℃.
Tom qab ob peb qhov rov qab siv dua, yog tias cov tshuaj tiv thaiv tau txo qis me ntsis, CALB tuaj yeem ntxiv kom tsim nyog thiab siv txuas ntxiv mus.Yog tias cov tshuaj tiv thaiv kev ua haujlwm raug txo qis, nws yuav tsum tau hloov.
Piv txwv 1 (Aminolysis)(1):
Piv txwv 2 (Aminolysis)(2):
Piv txwv 3 (Ntiv nplhaib qhib polyester synthesis)(3):
Piv txwv 4 (Transesterification, regioselective ntawm hydroxyl pawg)(4):
Piv txwv 5 (Transesterification, kinetic daws teeb meem ntawm racemic cawv)(5):
Piv txwv 6 (Esterification, kinetic daws teeb meem ntawm carboxylic acid)(6):
Piv txwv 7 (Esterolysis, kinetic daws)(7):
Piv txwv 8 (Hydrolysis ntawm amides)(8):
Piv txwv 9 (Acylation ntawm amines)(9):
Piv txwv 10 (Aza-Michael ntxiv cov tshuaj tiv thaiv)(10):
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