CALB uas tsis txav tau
CALB raug immobilized los ntawm kev nqus lub cev ntawm cov resin hydrophobic uas yog macroporous, styrene/methacrylate polymer. Immobilized CALB yog tsim rau kev siv hauv cov organic solvents thiab cov solvent-free systems, thiab tuaj yeem rov ua dua tshiab thiab rov siv dua ntau zaus hauv cov xwm txheej tsim nyog.
Product Code: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★Kev ua haujlwm siab dua, kev xaiv chiral siab dua thiab kev ruaj khov siab dua.
★Kev ua tau zoo dua nyob rau hauv cov theem uas tsis yog dej.
★Yooj yim tshem tawm ntawm lub cev ua tshuaj, ua kom cov tshuaj tsis ua haujlwm sai sai, thiab zam cov protein seem hauv cov khoom.
★ Rov siv dua thiab siv dua kom txo tus nqi.
| Kev Ua Si | ≥10000PLU/g |
| pH ntau rau kev ua tshuaj tiv thaiv | 5-9 |
| Qhov kub thiab txias rau kev ua tshuaj tiv thaiv | 10-60 ℃ |
| Qhov tsos | CALB-IMMO100-A: Xim av daj mus rau xim av daj CALB-IMMO100-B: Dawb mus rau xim av daj khov kho |
| Qhov loj ntawm cov khoom me me | 300-500μm |
| Poob thaum ziab ntawm 105 ℃ | 0.5% -3.0% |
| Resin rau kev immobilization | Macroporous, styrene/methacrylate polymer |
| Cov tshuaj tiv thaiv | Dej, cov kuab tshuaj organic, thiab lwm yam, lossis tsis muaj kuab tshuaj. Rau cov tshuaj tiv thaiv hauv qee cov kuab tshuaj organic, 3% dej tuaj yeem ntxiv los txhim kho cov nyhuv tshuaj tiv thaiv |
| Qhov loj ntawm cov khoom me me | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Kev txhais lus ntawm chav tsev: 1 chav tsev sib raug rau kev tsim cov 1μmol ib feeb propyl laurate los ntawm lauric acid thiab 1-propanol ntawm 60 ℃. Cov CALB-IMMP100-A thiab CALB-IMMO100-B saum toj no sib raug rau cov neeg nqa khoom immobilized nrog cov qhov loj sib txawv.
1. Hom tshuaj tiv thaiv
Cov enzyme immobilized siv tau rau ob qho tib si kettle batch reactor thiab fixed bed continuous flow reactor. Nws yuav tsum tau sau tseg kom tsis txhob muaj kev sib tsoo vim yog lub zog sab nraud thaum pub mis lossis sau.
2. Cov pH ntawm cov tshuaj tiv thaiv, qhov kub thiab cov kuab tshuaj
Cov enzyme uas tsis txav tau yuav tsum tau ntxiv rau qhov kawg, tom qab ntxiv thiab yaj lwm cov ntaub ntawv, thiab kho pH.
Yog tias kev siv cov substrate lossis kev tsim cov khoom yuav ua rau muaj kev hloov pauv ntawm pH thaum lub sijhawm ua haujlwm, yuav tsum ntxiv cov buffer txaus rau hauv lub kaw lus ua haujlwm, lossis yuav tsum saib xyuas thiab kho pH thaum lub sijhawm ua haujlwm.
Hauv qhov kub thiab txias ntawm CALB (qis dua 60 ℃), qhov hloov pauv nce ntxiv nrog rau qhov kub thiab txias nce ntxiv. Hauv kev siv tiag tiag, qhov kub ntawm cov tshuaj tiv thaiv yuav tsum tau xaiv raws li qhov ruaj khov ntawm cov khoom siv lossis cov khoom.
Feem ntau, cov tshuaj ester hydrolysis reaction yog tsim nyog rau hauv aqueous phase system, thaum cov tshuaj ester synthesis reaction yog tsim nyog rau hauv organic phase system. Cov organic solvent tuaj yeem yog ethanol, tetrahydrofuran, n-hexane, n-heptane thiab toluene, lossis cov tshuaj sib xyaw ua ke. Rau cov tshuaj tiv thaiv hauv qee cov organic solvents, 3% dej tuaj yeem ntxiv rau kom txhim kho cov nyhuv tshuaj tiv thaiv.
3. Siv dua thiab lub neej ua haujlwm ntawm CALB
Nyob rau hauv qhov xwm txheej tsim nyog, CALB tuaj yeem rov qab tau thiab siv dua, thiab lub sijhawm thov tshwj xeeb sib txawv nrog cov haujlwm sib txawv.
Yog tias CALB uas tau rov qab los tsis tau siv dua tas li thiab yuav tsum tau khaws cia tom qab rov qab los, nws yuav tsum tau ntxuav thiab ziab thiab kaw rau ntawm 2-8 ℃.
Tom qab siv ob peb zaug, yog tias qhov ua tau zoo ntawm cov tshuaj tiv thaiv txo qis me ntsis, CALB tuaj yeem ntxiv rau kom raug thiab txuas ntxiv siv. Yog tias qhov ua tau zoo ntawm cov tshuaj tiv thaiv txo qis heev, nws yuav tsum tau hloov.
Piv txwv 1 (Aminolysis)(1):
Piv txwv 2 (Aminolysis)(2):
Piv txwv 3 (Kev tsim cov polyester qhib lub nplhaib)(3):
Piv txwv 4 (Kev hloov pauv esterification, regioselective ntawm hydroxyl pawg)(4):
Piv txwv 5 (Kev Hloov Pauv, kev daws teeb meem kinetic ntawm cov cawv racemic)(5):
Piv txwv 6 (Esterification, kinetic resolution ntawm carboxylic acid)(6):
Piv txwv 7 (Esterolysis, kinetic resolution)(7):
Piv txwv 8 (Kev sib cais cov amides)(8):
Piv txwv 9 (Acylation ntawm amines)(9):
Piv txwv 10 (Kev sib ntxiv ntawm Aza-Michael)(10):
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5. Kamble MP, Shinde SD, Yadav G DJ Mol. Catal. B: Enzym, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. Txheej Txheem Biochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, e tal. J. Mol. Catal. B: Enzym, 2016, 130:58-69.
8. Gavi´an AT, Castillo E, L´opez-Mungu´AJ Mol. Catal. B: Enzym, 2006, 41: 136-140.
9. Joubioux FL, Henda YB, Bridiau N, e tal. J. Mol. Catal. B: Enzym, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, et al. Tetrahedron Lett, 2010, 51: 4455-4458.
