Lactate Dehydrogenase (LDH)
Lus piav qhia luv luv:
Hais txog Lactate dehydrogenase
Muaj 1 yam khoom LDH enzyme (Number as ES-LDH) tsim los ntawm SyncoZymes.LDH tuaj yeem ua rau txo qis ntawm pyruvate rau L-lactic acid, lossis txo qis ntawm lwm yam zoo li α-keto acids.Nyob rau hauv tus txheej txheem ntawm catalysis, NADH yog tsim nyog raws li hydrogen transporter.
Lactate dehydrogenasecatalytic cov tshuaj tiv thaiv hom
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E-Mail:lchen@syncozymes.com
| Enzymes | Khoom Code | Specification |
| Enzyme hmoov | ES-LDH-101 | ib txheej ntawm 1 Monoamine Oxidase, 50 mg txhua 1 yam khoom * 50mg / khoom, lossis lwm yam |
Cov tshuaj tiv thaiv hauv qab no tuaj yeem siv rau kev siv:
1-10 mg/ml: substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate tsis kam (pH7.0)
0.2 mg / ml: NAD +
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate cov tshuaj tiv thaiv ntawm qhov ntsuas kub tsis tu ncua (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev.Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Lus Cim (1): Organic hnyav (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov substrates insoluble.
