Leucine Dehydrogenase (LeuDH)
Lus piav qhia luv luv:
Hais txog Leucine dehydrogenase
Muaj 1 hom LeuDH enzyme khoom (Ntau li ES-LeuDH-101) tsim los ntawm SyncoZymes.LeuDH tuaj yeem ua rau txo qis amoniation ntawm 4-methylbutanoic acid (ntsev) rau L-leucine lossis oxidation ntawm leucine rau 4-methylbutanoic acid.ES-LeuDH kuj tseem tuaj yeem ua rau txo qis ntawm α-keto acids nrog cov qauv zoo sib xws los npaj cov amino acid derivatives sib xws.Nyob rau hauv tus txheej txheem ntawm catalysis, NADH yog tsim nyog raws li hydrogen transporter.
Leucine dehydrogenasecatalytic cov tshuaj tiv thaiv hom
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E-Mail:lchen@syncozymes.com
| Enzymes | Khoom Code | Specification |
| Enzyme hmoov | ES-LeuDH-101 | ib txheej ntawm 1 Monoamine Oxidase, 50 mg txhua 1 yam khoom * 50mg / khoom, lossis lwm yam |
Cov tshuaj tiv thaiv hauv qab no tuaj yeem siv rau kev siv:
1-10 mg/ml: substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate tsis kam (pH7.0)
0.2 mg / ml: NAD +
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate cov tshuaj tiv thaiv ntawm qhov ntsuas kub tsis tu ncua (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev.Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Lus Cim (1): Organic hnyav (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov substrates insoluble.
