Leucine Dehydrogenase (LeuDH)
Kev Piav Qhia Luv:
Txog Leucine dehydrogenase
Muaj 1 hom khoom LeuDH enzyme (Tus lej ua ES-LeuDH-101) tsim los ntawm SyncoZymes. LeuDH tuaj yeem ua rau cov ammoniation ntawm 4-methylbutanoic acid (ntsev) ua L-leucine lossis oxidation ntawm leucine ua 4-methylbutanoic acid. ES-LeuDH kuj tseem tuaj yeem ua rau cov amination ntawm α-keto acids uas muaj cov qauv zoo sib xws los npaj cov amino acid derivatives. Hauv cov txheej txheem ntawm catalysis, NADH yog qhov tsim nyog ua tus thauj hydrogen.
Leucine dehydrogenasecatalytic reaction hom
Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526
Email:lchen@syncozymes.com
| Cov Enzymes | Cov Cai Khoom | Cov Lus Qhia Tshwj Xeeb |
| Hmoov Enzyme | ES-LeuDH-101 | ib pawg ntawm 1 Monoamine Oxidase, 50 mg txhua yam khoom * 50mg / yam khoom, lossis lwm yam kom muaj nuj nqis |
Cov txheej txheem tshuaj tiv thaiv hauv qab no siv tau rau kev siv ua piv txwv:
1-10 mg/ml: Cov khoom siv
10 mg/ml: Cov Enzyme
10% (v/v): Cov tshuaj organic (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Muab cov tshuaj tiv thaiv tso rau ntawm lub tshuab co qhov kub tas li (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev. Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC kev tshuaj xyuas.
Lus Cim (1): Cov kuab tshuaj organic (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov khoom tsis yaj.
