Nitrile hydratase (NHT)

Kev Piav Qhia Luv:

Txog ES-NHTs

ES-NHTs: ib chav kawm ntawm metalloenzyme, uas xaiv hydrate nitriles rau hauv cov thawj amide sib xws. Lawv siv dav tsis yog rau kev tsim cov amino acids, amides, carboxylic acids thiab lawv cov derivatives, tab sis kuj rau kev npaj cov tshuaj chiral. Muaj 24 hom nitrile hydratases tsim los ntawm SyncoZymes (Tus lej li ES-NHT-101 ～ ES-NHT-124).
Hom tshuaj tiv thaiv catalytic:

Nitrile hydratase NHT2

Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526

Email:lchen@syncozymes.com

Xa email rau peb

Cov Khoom Qhia Txog Khoom

Cov Cim Npe Khoom

Cov Ntaub Ntawv Khoom:

Cov Enzymes	Cov Cai Khoom	Cov Lus Qhia Tshwj Xeeb
Hmoov Enzyme	ES-NHT-101~ ES-NHT-124	ib pawg ntawm 24 Nitrile Hydratases, 50 mg txhua 24 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas
Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit)	ES-NHT-2400	ib pawg ntawm 24 Nnitrile Hydratases, 1 mg txhua 24 yam khoom * 1mg / yam khoom

Cov txiaj ntsig:

★ Kev tshwj xeeb ntawm cov substrate siab.
★ Muaj zog xaiv chiral.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.

Cov lus qhia rau kev siv:

➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov substrate, cov kua buffer (qhov pH zoo tshaj plaws ntawm cov tshuaj tiv thaiv) thiab ES-NHT.
➢ Tag nrho cov ES-NHTs tuaj yeem kuaj xyuas tau hauv lub kaw lus teb saum toj no lossis nrog NHT Screening Kit (SynKit NHT).
➢ Yuav tsum kawm txog txhua yam ES-NHTs uas sib raug rau ntau yam xwm txheej zoo tshaj plaws ntawm kev teb.
➢ Cov khoom siv uas muaj cov concentration siab heev yuav ua rau ES-NHT tsis ua haujlwm. Txawm li cas los xij, qhov kev txwv no yuav raug txo los ntawm kev ntxiv cov khoom siv ntau ntau, sib txuas nrog cov amidases lossis rho tawm cov khoom.

Piv txwv ntawm daim ntawv thov:

Piv txwv 1 (kev sib txuas nrog amidase)⁽¹⁾:

Piv txwv 2⁽²⁾:

Kev Khaws Cia:

Khaws cia rau 2 xyoos hauv qab -20 ℃.

Ceeb Toom:

Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab / qis thiab cov kuab tshuaj organic siab.

Cov ntaub ntawv siv los ua piv txwv:

1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71:51-55.
2 Gary W. B, Thomas G, Christopher B. M, et al. Tetrahedron lett, 2010, 51: 1639-1641.

Yav dhau los: Nitrilase (NIT)

Tom ntej no: Nitrile reductase (NRED)

Sau koj cov lus ntawm no thiab xa tuaj rau peb