Nitrile reductase (NRED)
Lus piav qhia luv luv:
Hais txog Nitrile reductase
Muaj 3 yam khoom NRED enzyme (Ntau li ES-NRED101 ~ ES-NRED103) tsim los ntawm SyncoZymes.NRED tuaj yeem ua rau txo qis ntawm pawg nitrile rau amine.Nyob rau hauv tus txheej txheem ntawm catalysis, NADPH yog xav tau raws li hydrogen transporter.
Nitrile reductasecatalytic cov tshuaj tiv thaiv
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| Enzymes | Khoom Code | Specification |
| Specification | ES- NRED -101 ~ ES- NRED -103 | ib txheej ntawm 3 Nitrile Reductase, 50 mg txhua 3 yam khoom * 50mg / khoom, lossis lwm yam |
Cov tshuaj tiv thaiv hauv qab no tuaj yeem siv rau kev siv:
1-10 mg / ml substrate
10 mg/ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate tsis kam (pH7.0)
0.2 mg/ml NADP +
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Incubate cov tshuaj tiv thaiv ntawm qhov ntsuas kub tsis tu ncua (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev.Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Lus Cim (1): Organic hnyav (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov substrates insoluble.
