Ene reductase (ERED)
ES-EREDs catalyze ntau hom substrate vim muaj dav spectrum ntawm substrates.Feem ntau, C = C ntawm α, β -unsaturated compounds nrog electron-absorbing pawg (xws li ketone aldehyde, nitro pawg, carboxylic acids, esters, anhydride, lactones, imines, thiab lwm yam) tau yooj yim txo los ntawm ES-EREDs, tab sis. lub unactivated ob daim ntawv cog lus tsis.
Muaj 46 hom ERED enzyme khoom (Ntau li ES-ERED-101 ~ ES-ERED-146) tsim los ntawm SyncoZymes.
Catalytic mechanism:
| Enzymes | Khoom Code | Specification |
| Enzyme hmoov | ES-ERED-101 ~ ES-ERED-146 | ib txheej ntawm 46 Ene Reductases, 50 mg txhua 46 yam khoom * 50mg / khoom, lossis lwm yam |
| Cov khoom siv tshuaj ntsuam xyuas (SynKit) | ES-ERED-4600 | ib txheej ntawm 46 Ene Reductases, 50 mg txhua 46 yam khoom * 50mg / khoom, lossis lwm yam |
★ High substrate specification.
★ Muaj zog chiral selectivity.
★ Kev hloov pauv siab.
★ Tsawg los ntawm cov khoom lag luam.
★ Cov tshuaj tiv thaiv me me.
★ Ib puag ncig tus phooj ywg.
★ Kev nyab xeeb siab.
➢ Nquag, cov txheej txheem tshuaj tiv thaiv yuav tsum suav nrog substrate, tsis muaj tshuaj (pH zoo tshaj plaws), coenzymes (NAD(H) lossis NADP(H)), coenzyme regeneration system (xws li qabzib thiab qabzib dehydrogenase) thiab ES-ERED.
➢ Tag nrho cov ES-ERED tuaj yeem kuaj tau raws li hauv cov txheej txheem tshuaj tiv thaiv saum toj no lossis nrog ERED Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit ERED).
➢ Txhua yam ntawm ES-EREDs sib haum rau ntau yam kev xav tau zoo yuav tsum tau kawm ib tus zuj zus.
➢ High concentration Substrate los yog cov khoom uas muaj peev xwm inhibit ES-ERED txoj haujlwm.Txawm li cas los xij, qhov inhibition tuaj yeem daws tau los ntawm batch ntxiv ntawm substrate.
Piv txwv 1 (α, beta-unsaturated aldehydes los yog ketones)(1):
Piv txwv 2 (α, β-unsaturated carboxylic acids thiab lawv cov derivatives)(2):
Khaws 2 xyoos qis dua -20 ℃.
Tsis txhob tiv tauj nrog huab cua xws li: kub siab, siab / qis pH thiab siab concentration organic hnyav.
1. Lucídio C, Fardelone J, Augusto R, thiab tal.J.Mol.Catal.B:Enzym., 2004, 29:41-45.
2. Stueckler C, Hall M, Ehammer H, thiab tal..Org.Lett, 2007, 9(26): 5409-5411.
