Nucloside phosphoryalse (NP)

Kev Piav Qhia Luv:

Txog nucleoside phosphoryalse

Muaj 3 hom khoom NP enzyme (Tus lej li ES-NP-101 ~ ES-NP-103) tsim los ntawm SyncoZymes. ES-NP-101 yog purine nucleoside phosphorylase, ES-NP-102 thiab ES-NP-103 yog pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase tuaj yeem rhuav tshem nucleosides mus rau hauv cov hauv paus thiab pentose phosphate. Nucleoside phosphorylase tuaj yeem faib ua purine nucleoside phosphorylase thiab pyrimidine nucleoside phosphorylase raws li qhov nyiam ntawm cov hauv paus nucleoside. Purine nucleoside phosphorylase tuaj yeem hloov adenosine mus rau adenine, inosine mus rau hypoxanthine, guanosine mus rau guanine, thiab tsim ribose phosphate tib lub sijhawm. Pyrimidine nucleoside phosphorylase tuaj yeem hloov uridine mus rau uracil thiab tsim ribose phosphate.

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Nucloside phosphoryalse hom tshuaj tiv thaiv:

Cov Ntaub Ntawv Khoom:

Cov Enzymes	Cov Cai Khoom	Cov Lus Qhia Tshwj Xeeb
Hmoov Enzyme	ES-NP-101~ ES-NP-103	ib pawg ntawm 3 Nuclosidephosphoryalse, 50 mg txhua 3 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas

Cov txheej txheem tshuaj ntsuam xyuas phosphoryalse ntawm nucloside:

Cov txheej txheem tshuaj tiv thaiv hauv qab no siv tau rau kev siv ua piv txwv:
1-10 mg / ml Cov Khoom Siv
10 mg/ml Enzyme
100 mM Phosphate buffer (pH7.0)
Muab cov tshuaj tiv thaiv tso rau ntawm lub tshuab co qhov kub tas li (piv txwv li, 30oC, 150rpm) rau 12 ~ 24 teev. Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC kev tshuaj xyuas.