Nucloside phosphoryalse (NP)
Enzymes | Khoom Code | Specification |
Enzyme hmoov | ES-NP-101 ~ ES-NP-103 | ib txheej ntawm 3 Nuclosidephosphoryalse, 50 mg txhua 3 yam khoom * 50mg / khoom, lossis lwm yam |
Cov tshuaj tiv thaiv hauv qab no tuaj yeem siv rau kev siv:
1-10 mg / ml substrate
10 mg/ml Enzyme
100 mM Phosphate tsis kam (pH7.0)
Incubate cov tshuaj tiv thaiv ntawm qhov ntsuas kub tsis tu ncua (piv txwv li, 30oC, 150rpm) rau 12 ~ 24 teev.Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Sau koj cov lus ntawm no thiab xa tuaj rau peb