Transaminase (ATA)
Enzymes: Yog macromolecular lom catalysts, feem ntau enzymes yog cov proteins.
Transaminases: Ib chav kawm ntawm enzymes uas catalyze amino hloov ntawm amino acids thiab keto acids.Transaminases yog cov enzymes tseem ceeb hauv asymmetric synthesis thiab racemic daws ntawm chiral amines.
Aminotransferase tuaj yeem muab faib ua plaub chav kawm raws li qib thiab cov qauv: Ⅰ, Ⅱ, Ⅲ thiab Ⅳ.ω-aminotransferases yog nyob rau hauv chav kawm II transaminases, feem ntau yog siv rau hauv kev npaj ntawm chiral amines thiab unnatural amino acids, xws li β-amino acids.
ω-aminotransferases: Feem ntau, ω-transaminase yog hais txog ib chav kawm ntawm enzymes, uas catalytic ammonia hloov cov tshuaj tsis muaj α-amino acid li substrate lossis khoom.
Catalytic mechanism:
| Enzymes | Khoom Code | Khoom Code |
| Enzyme hmoov | ES-ATA-101 ~ ES-ATA-165 | ib txheej ntawm 65 ω-Transaminases, 50 mg txhua 65 yam khoom * 50mg / khoom, lossis lwm yam |
| Cov khoom siv tshuaj ntsuam xyuas (SynKit) | ES-ATA-6500 | ib txheej ntawm 65 ω-Transaminases, 1 mg txhua 65 yam khoom * 1mg / khoom |
★ High substrate specification.
★ Muaj zog chiral selectivity.
★ High conversion efficiency.
★ Tsawg los ntawm cov khoom lag luam.
★ Cov tshuaj tiv thaiv me me.
★ Ib puag ncig tus phooj ywg.
➢ Kev tshuaj xyuas Enzyme yuav tsum tau ua rau cov substrates tshwj xeeb vim yog cov substrate tshwj xeeb, thiab tau txais ib qho enzyme uas catalyzes lub hom phiaj substrate nrog cov nyhuv catalytic zoo tshaj plaws.
➢ Tsis txhob tiv tauj nrog cov xwm txheej hnyav xws li: qhov kub thiab txias, pH siab / qis thiab cov kuab tshuaj organic nrog siab concentration.
➢ Nquag, cov tshuaj tiv thaiv yuav tsum muaj xws li substrate, buffer tov, amino pub (xws li amino acids thiab 1-phenyl ethylamine) los yog receptor (xws li keto acids), coenzyme (PLP), cosolvent (xws li DMSO).
➢ ATA yuav tsum tau muab ntxiv rau qhov kawg ntawm cov tshuaj tiv thaiv kab mob, tom qab pH thiab kub tau hloov kho rau cov tshuaj tiv thaiv.
➢ Txhua yam ntawm ATA muaj ntau yam kev pom zoo, yog li txhua tus ntawm lawv yuav tsum tau kawm ntxiv ib tus zuj zus.
Piv txwv 1 (synthesis ntawm Sitagliptin, asymmetric synthesis)(1):
Piv txwv 2 (Mexiletine, Kev sib xyaw ntawm kinetic daws nrog asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, thiab al.Organic Letters, 2009, 11(21): 4810-4812.
