Transaminase (ATA)
Cov Enzymes: Yog cov catalysts macromolecular biological, feem ntau cov enzymes yog cov protein.
Transaminases: Ib pawg ntawm cov enzymes uas catalyze amino hloov pauv ntawm amino acids thiab keto acids. Transaminases yog cov enzymes tseem ceeb hauv kev tsim cov asymmetric thiab racemic daws teeb meem ntawm chiral amines.
Aminotransferase muaj peev xwm muab faib ua plaub pawg raws li qhov sib lawv liag thiab cov qauv: Ⅰ, Ⅱ, Ⅲ thiab Ⅳ. ω-aminotransferases koom nrog pawg II transaminases, feem ntau siv rau hauv kev npaj cov chiral amines thiab cov amino acids tsis zoo li qub, xws li β-amino acids.
ω-aminotransferases: Feem ntau, ω-transaminase yog hais txog ib chav kawm ntawm cov enzymes, uas catalytic ammonia hloov pauv cov tshuaj tiv thaiv tsis muaj α-amino acid ua cov khoom siv lossis cov khoom.
Cov txheej txheem catalytic:
| Cov Enzymes | Cov Cai Khoom | Cov Cai Khoom |
| Hmoov Enzyme | ES-ATA-101~ ES-ATA-165 | ib pawg ntawm 65 ω-Transaminases, 50 mg txhua 65 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas |
| Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit) | ES-ATA-6500 | ib pawg ntawm 65 ω-Transaminases, 1 mg txhua 65 yam khoom * 1mg / yam khoom |
★ Kev tshwj xeeb ntawm cov substrate siab.
★ Muaj zog xaiv chiral.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.
➢ Yuav tsum tau kuaj xyuas enzyme rau cov substrates tshwj xeeb vim yog qhov tshwj xeeb ntawm cov substrate, thiab tau txais cov enzyme uas ua rau lub substrate catalytic zoo tshaj plaws.
➢ Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab/qis thiab cov kuab tshuaj organic uas muaj concentration siab.
➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov substrate, buffer solution, amino donor (xws li amino acids thiab 1-phenyl ethylamine) lossis receptor (xws li keto acids), coenzyme (PLP), cosolvent (xws li DMSO).
➢ Yuav tsum ntxiv ATA kawg rau hauv qhov system tshuaj tiv thaiv, tom qab pH thiab qhov kub tau kho rau qhov xwm txheej tshuaj tiv thaiv.
➢ Txhua hom ATA muaj ntau yam kev mob tshwm sim zoo tshaj plaws, yog li txhua yam ntawm lawv yuav tsum tau kawm ntxiv.
Piv txwv 1 (kev tsim cov Sitagliptin, kev tsim cov tshuaj tsis sib xws)(1):
Piv Txwv 2 (Mexiletine, Kev Sib Xyaws ntawm kev daws teeb meem kinetic nrog kev sib xyaw ua ke tsis sib xws)(2):
1 Savile CK, Janey JM, Mundorff EC, thiab lwm tus. Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, thiab al. Organic Letters, 2009, 11(21): 4810-4812.
