Cawv oxidase (AOX)
Kev Piav Qhia Luv:
Txog cawv oxidase
ES-AOXs: Cov enzymes no muaj peev xwm ua kom cov roj alcohols lossis aryl-alcohols ua aldehydes. Lawv siv cov pa oxygen molecular tab sis tsis xav tau cov cofactor ntxiv sab nraud, thiab hydrogen peroxide tsim tawm.
Muaj 6 hom cawv oxidases khoom (Tus lej li ES-AOX-101 ~ ES-AOX-106) tsim los ntawm SyncoZymes. ES-AOX101 thiab ES-AOX102 nyiam aliphatic cawv substrates, ES-AOX103 ~ ES-AOX105 nyiam aromatic cawv substrates, thiab ES-AOX106 yog cholesterol oxidase. SZ-AOX yog ib qho cuab yeej pab tau los catalyze oxidation ntawm fatty cawv lossis aryl-alcohols los tsim aldehydes.
Hom tshuaj tiv thaiv catalytic:
Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526
Email:lchen@syncozymes.com
| Cov Enzymes | Cov Cai Khoom | Cov Lus Qhia Tshwj Xeeb |
| Hmoov Enzyme | ES-AOX-101~ ES-AOX-105 | ib pawg ntawm 5 cov cawv oxidases, 50 mg txhua 5 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas |
| Cov Khoom Siv Tshuaj Ntsuam Xyuas (SynKit) | ES-AOX-500 | ib pawg ntawm 5 cov cawv oxidases, 50 mg txhua 5 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas |
★ Kev tshwj xeeb ntawm cov substrate siab.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.
➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov khoom siv substrate, cov kua buffer thiab ES-AOX, thiab yuav tsum muaj oxygen.
➢ Txhua yam ES-AOXs sib raug rau ntau yam kev mob zoo tshaj plaws, uas tuaj yeem kawm tau ib leeg.
➢ Cov khoom siv uas muaj cov tshuaj ntau dhau los yog cov khoom uas muaj cov tshuaj no yuav ua rau ES-AOX tsis ua haujlwm. Txawm li cas los xij, qhov kev txwv no yuav raug txo los ntawm kev ntxiv cov tshuaj ntxiv rau hauv.
➢ Kev sib sau ua ke ntawm H2O2nyob rau hauv lub cev yuav ua rau enzyme inactivation, uas tuaj yeem daws tau zoo los ntawm kev siv catalase.
Piv txwv 1 (Kev sib xyaw ua ke ntawm aryl-alcohols)(1):
Piv txwv 2 (Kev ua kom cov cawv rog tawm)(2):
Khaws cia rau 2 xyoos hauv qab -20 ℃.
Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab / qis thiab cov kuab tshuaj organic siab.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, et tal. J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, et al. Appl Microbiol Biot, 1992, 37:66-73.
