Esterase & Lipase (PLE & CALB)
Muaj 26 hom khoom PLE enzyme (Tus lej li ES-PLE-101 ～ ES-PLE-126) tsim los ntawm SyncoZymes. ES-PLE siv tau rau hydrolysis ntawm aliphatic thiab ester compounds, lossis regioselective thiab stereoselective kev daws teeb meem los tsim cov chiral acids thiab lawv cov derivatives.
Hom tshuaj tiv thaiv catalytic:
★ Kev tshwj xeeb ntawm cov substrate siab.
★ Muaj zog xaiv chiral.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.
➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov substrate, cov kua buffer thiab ES-PLE. Kev esterification ntawm qee cov ES-PLEs yog ua tiav hauv theem organic.
➢ Yuav tsum kawm txog txhua yam ES-PLEs uas sib raug rau ntau yam xwm txheej zoo tshaj plaws ntawm kev teb.
Piv txwv 1 (Kev tsim cov tshuaj Pregabalin Intermediate)(1):
Piv txwv 2(2):
Piv txwv 3(3):
Piv txwv 4(4):
Khaws cia rau 2 xyoos hauv qab -20 ℃.
Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab / qis.
1. Xu FX, Chen SY, Xu G, thiab lwm yam. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD et tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
