Nitrile reductase (NRED)
Kev Piav Qhia Luv:
Txog Nitrile reductase
Muaj 3 hom khoom NRED enzyme (Tus lej ua ES-NRED101 ~ ES-NRED103) tsim los ntawm SyncoZymes. NRED tuaj yeem ua rau pawg nitrile txo qis mus rau amine. Hauv cov txheej txheem ntawm catalysis, NADPH yog qhov xav tau ua tus thauj hydrogen.
Hom tshuaj tiv thaiv Nitrile reductasecatalytic
Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526
Email:lchen@syncozymes.com
| Cov Enzymes | Cov Cai Khoom | Cov Lus Qhia Tshwj Xeeb |
| Cov Lus Qhia Tshwj Xeeb | ES- NRED -101~ ES- NRED -103 | ib pawg ntawm 3 Nitrile Reductase, 50 mg txhua yam 3 yam khoom * 50mg / yam khoom, lossis lwm yam kom muaj nuj nqis |
Cov txheej txheem tshuaj tiv thaiv hauv qab no siv tau rau kev siv ua piv txwv:
1-10 mg / ml Cov Khoom Siv
10 mg/ml Enzyme
10% (v/v) Cov tshuaj organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Qab Zib
2 mg/ml Glucose dehydrogenase (GDH)
Muab cov tshuaj tiv thaiv tso rau ntawm lub tshuab co qhov kub tas li (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev. Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC kev tshuaj xyuas.
Lus Cim (1): Cov kuab tshuaj organic (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov khoom tsis yaj.
