Hydroaminase (HAM)
Kev Piav Qhia Luv:
Txog Hydroaminase
Muaj 2 hom khoom HAM enzyme (Tus lej ua ES-HAM-101 ~ ES-HAM-102) tsim los ntawm SyncoZymes. HAM tuaj yeem ua rau cov amino acids enoic lossis nws cov derivatives ua chiral amino acids. HAM tuaj yeem siv los ua cov chiral amino acids (lossis chiral amines) los ntawm enoic acids (lossis alkenes). Cov neeg pub ammonia, xws li dej ammonia lossis ntsev ammonium, yog qhov tsim nyog.
Hom tshuaj tiv thaiv catalytic hydroaminase
Xov tooj ntawm tes/Wechat/WhatsApp: +86-13681683526
Email:lchen@syncozymes.com
| Cov Enzymes | Cov Cai Khoom | Cov Lus Qhia Tshwj Xeeb |
| Hmoov Enzyme | ES-HAM-101~ ES-HAM-102 | ib pawg ntawm 2 Nuclosidephosphoryalse, 50 mg txhua 2 yam khoom * 50mg / yam khoom, lossis lwm yam ntau npaum li cas |
Cov txheej txheem tshuaj tiv thaiv hauv qab no siv tau rau kev siv ua piv txwv:
1-10 mg/ml: Cov khoom siv
10 mg/ml: Cov Enzyme
10% (v/v): Cov tshuaj organic (1)
10% (v/v): Ammonia
Kho pH rau 9.0 ua ntej ntxiv enzyme, thiab thaum kawg yuav tsum ntxiv enzyme. Incubate cov tshuaj tiv thaiv ntawm lub shaker kub tas li (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev. Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Lus Cim (1): Cov kuab tshuaj organic (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov khoom tsis yaj.
