Hydroaminase (HAM)
Lus piav qhia luv luv:
Hais txog Hydroaminase
Muaj 2 hom HAM enzyme khoom (Ntau li ES-HAM-101 ~ ES-HAM-102) tsim los ntawm SyncoZymes.HAM tuaj yeem ua rau cov ammonia ntawm enoic acid los yog nws cov derivatives los tsim chiral amino acids.HAM tuaj yeem siv los tsim cov chiral amino acids (los yog chiral amines) los ntawm enoic acids (los yog alkenes).Ammonia pub, xws li dej ammonia lossis ammonium ntsev, yog qhov tsim nyog.
Hydroaminase catalytic cov tshuaj tiv thaiv hom
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| Enzymes | Khoom Code | Specification |
| Enzyme hmoov | ES-HAM-101 ~ ES-HAM-102 | ib txheej ntawm 2 Nuclosidephosphoryalse, 50 mg txhua 2 yam khoom * 50mg / khoom, lossis lwm yam |
Cov tshuaj tiv thaiv hauv qab no tuaj yeem siv rau kev siv:
1-10 mg/ml: substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
10% (v/v): Ammonia
Kho pH rau 9.0 ua ntej ntxiv enzyme, thiab enzyme yuav tsum tau ntxiv thaum kawg.Incubate cov tshuaj tiv thaiv ntawm qhov ntsuas kub tsis tu ncua (piv txwv li, 30oC, 150rpm) rau 24 ~ 48 teev.Centrifuge txhua qhov sib tov kom precipitate protein, lawv cov supernatant tuaj yeem siv rau TLC, HPLC lossis GC tsom xam.
Lus Cim (1): Organic hnyav (xws li DMSO, methanol, acetonitrile, IPA, thiab lwm yam) yog xaiv tau, siv rau cov substrates insoluble.
