Phenylalanine dehydrogenase (PDH)
Kev Piav Qhia Luv:
Txog Phenylalanine dehydrogenase
ES-PDH: Ib chav kawm ntawm oxidoreductase. Qhov kev tshuaj tiv thaiv zoo tuaj yeem ua rau cov ntsev L phenylalanine oxidative deamination nyob rau hauv qhov muaj NAD los tsim cov ntsev phenylpyruvate, thiab qhov kev tshuaj tiv thaiv rov qab tuaj yeem ua rau cov ntsev amino acid sib xws los ntawm cov ntsev phenylpyruvate. Syncozymes tau tsim 8 yam khoom ntawm phenylalanine dehydrogenase (suav nrog ES-PDH-101 ~ ES-PDH-108). ES-PDH tuaj yeem siv los npaj cov ntsev phenylalanine lossis cov ntsev amino acid sib xws los ntawm cov ntsev phenylpyruvic acid lossis cov khoom siv.
Hom tshuaj tiv thaiv catalytic:
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Email:lchen@syncozymes.com
| Cov Enzymes | Cov Cai Khoom | Cov Lus Qhia Tshwj Xeeb |
| Hmoov Enzyme | ES-PDH-101~ ES-PDH-108 | ib pawg ntawm 8 Nitrile Reductase, 50 mg txhua 8 yam khoom * 50mg / yam khoom, lossis lwm yam kom muaj nuj nqis |
★ Kev tshwj xeeb ntawm cov substrate siab.
★ Muaj zog xaiv chiral.
★ Kev hloov pauv siab heev.
★ Cov khoom tsis muaj txiaj ntsig tsawg dua.
★ Cov mob ua xua me me.
★ Ib puag ncig zoo.
➢ Feem ntau, lub kaw lus tshuaj tiv thaiv yuav tsum muaj cov substrate, buffer solution, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (piv txwv li ammonium formate thiab formate dehydrogenase).
➢ Txhua hom ES-PDH sib raug rau ntau yam kev mob tshwm sim zoo tshaj plaws, uas tuaj yeem kawm ib leeg yog tias xav tau.
➢ Yuav tsum ntxiv ES-PDH rau hauv qhov system tshuaj tiv thaiv kom tswj tau qhov kev ua haujlwm.
Piv txwv 1 (Kev tsim cov phenylalanine derivatives los ntawm phenylpyruvate derivatives)(1):
Khaws cia rau 2 xyoos hauv qab -20 ℃.
Tsis txhob kov cov xwm txheej hnyav xws li: kub heev, pH siab / qis thiab cov kuab tshuaj organic siab.
1. Francesca Paradisi, Stuart Collins, thiab lwm tus. Phau ntawv Journal of Biotechnology, 2007, 128, 408–411.
